| Gene | Mpi |
| Chromosome | 9 |
| Deletion Start | 57,552,716 |
| Deletion End | 57,544,669 |
| Deletion Size | 8,048 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,552,113 | 57,552,134 | CTGCACATCAGTCCACTTTACC |
| TUP | 57,552,085 | 57,552,111 | TGAGACCAAAGGATCTGACACAAGCCA |
| TUR | 57,552,055 | 57,552,075 | GCTACAGAGATTCCTCCCAAC |
| TDF | 57,544,801 | 57,544,819 | CCGTGGTGGAGTGCTCTTC |
| TDP | 57,544,770 | 57,544,798 | TGCAGCCAATGAGAGTGTCTTACTGAAGA |
| TDR | 57,544,729 | 57,544,749 | ACAGCAGGCCCGGAATATCAG |
PCR Primers
| Primer | Sequence |
| SU | TACATACGGGTAAAAGAAGGC |
| SD | CCAAAGGCTGACTGCTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18072A-A2 | VGB6 | C57BL/6NTac | |
| 18072A-C3 | VGB6 | C57BL/6NTac | |
| 18072A-C12 | VGB6 | C57BL/6NTac | |
