| Gene | Mrrf |
| Chromosome | 2 |
| Deletion Start | 36,159,686 |
| Deletion End | 36,141,477 |
| Deletion Size | 18,210 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,142,310 | 36,142,333 | CAGAACCTTTGTGGGATGAATGAG |
| TUP | 36,142,334 | 36,142,358 | TGACAGGAAATGATGAGGCAGGCCT |
| TUR | 36,142,360 | 36,142,382 | CCCTGATACACCACTGCAAACAG |
| TDF | 36,158,774 | 36,158,796 | TGAAGGGTGTTAGGGTGATAGTG |
| TDP | 36,158,799 | 36,158,827 | TCAGTGTAATGCTTCCATTGACTCTGCCA |
| TDR | 36,158,835 | 36,158,854 | TGTGTCCGTGTACTGAGCAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGAGCAGGCATTTAGAC |
| SD | CTGAAATTCACTGCTGTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18073A-B9 | VGB6 | C57BL/6NTac | |
| 18073A-G3 | VGB6 | C57BL/6NTac | |
| 18073A-F6 | VGB6 | C57BL/6NTac | |
