| Gene | Pcmtd2 |
| Chromosome | 2 |
| Deletion Start | 181,855,368 |
| Deletion End | 181,842,402 |
| Deletion Size | 12,967 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,842,595 | 181,842,614 | CGTGCATCTACTCGGAGGTG |
| TUP | 181,842,616 | 181,842,638 | TGGAGGCTCTGGATCTGCAGCCT |
| TUR | 181,842,651 | 181,842,673 | CCAGTACCACTGCCCAAATTCAG |
| TDF | 181,855,220 | 181,855,240 | AGCCACCAGTGAACTTCCTTC |
| TDP | 181,855,242 | 181,855,263 | TCAGAGGGTATTGCGCCTCCCA |
| TDR | 181,855,333 | 181,855,354 | CCAGCAGTTTCCTCTGGGTATC |
PCR Primers
| Primer | Sequence |
| SU | GTGTGTGGGCTCATACATGC |
| SD | CAGAGGCCAGCAATAGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 135 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18074A-C6 | VGB6 | C57BL/6NTac | |
| 18074A-D10 | VGB6 | C57BL/6NTac | |
| 18074A-F11 | VGB6 | C57BL/6NTac | |
| 18074A-E3 | VGB6 | C57BL/6NTac | |
| 18074A-C10 | VGB6 | C57BL/6NTac | |
