| Gene | Pdcd2l |
| Chromosome | 7 |
| Deletion Start | 34,196,640 |
| Deletion End | 34,184,677 |
| Deletion Size | 11,964 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,196,138 | 34,196,156 | AGCCCTCACTGTGTCTGTC |
| TUP | 34,196,112 | 34,196,136 | TGCCTCTAAGCTGGAAGGTGTTCCG |
| TUR | 34,196,069 | 34,196,089 | CGCGTTCCAAGTCTCTTTCTC |
| TDF | 34,184,942 | 34,184,961 | GAAGGCAGGGCATGGGATAG |
| TDP | 34,184,915 | 34,184,941 | CCTAGGCCAGCTTGAACTGGCTATGTA |
| TDR | 34,184,882 | 34,184,903 | CCGGAGTGTCAGAAGTTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TACCGTCATCCTGTTTCCAG |
| SD | AGGAGGTGGTGGTTCAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18075A-B1 | VGB6 | C57BL/6NTac | |
| 18075A-C9 | VGB6 | C57BL/6NTac | |
| 18075A-F10 | VGB6 | C57BL/6NTac | |
| 18075A-F2 | VGB6 | C57BL/6NTac | |
| 18075A-F9 | VGB6 | C57BL/6NTac | |
