| Gene | Plcxd1 |
| Chromosome | 5 |
| Deletion Start | 110,103,667 |
| Deletion End | 110,100,226 |
| Deletion Size | 3,442 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,100,841 | 110,100,860 | ACAGCTCAGCAGGCCCATAG |
| TUP | 110,100,861 | 110,100,884 | TCACAGGCCTCCTGCCTCAGTTTC |
| TUR | 110,100,896 | 110,100,918 | ACATGGCAGGACTGAACAAGATG |
| TDF | 110,103,559 | 110,103,577 | GCAGGCGACTTCGTGGATG |
| TDP | 110,103,578 | 110,103,604 | CGGATGGCTTCGTGAGTAAGGTCATCA |
| TDR | 110,103,624 | 110,103,645 | GGGATGTGGCAGTTAGGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | GAAGGGAAGACCCATCACAG |
| SD | TGTCCTGTGTCTCCCTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18076B-E6 | VGB6 | C57BL/6NTac | |
| 18076B-G12 | VGB6 | C57BL/6NTac | |
| 18076A-D9 | VGB6 | C57BL/6NTac | |
