| Gene | Zwilch |
| Chromosome | 9 |
| Deletion Start | 64,172,867 |
| Deletion End | 64,139,912 |
| Deletion Size | 32,956 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,172,532 | 64,172,555 | TCAAGCTCAGAAACGGACAGAATC |
| TUP | 64,172,508 | 64,172,529 | CATCGACCCTCCTCGGCAGCCT |
| TUR | 64,172,478 | 64,172,496 | ACGGCTCACGCCTCTCATC |
| TDF | 64,140,026 | 64,140,045 | TGACTGGCTCGTTGTCTTTC |
| TDP | 64,140,000 | 64,140,025 | TGCTTGCAGAGTTTCCTGAGCTGACA |
| TDR | 64,139,971 | 64,139,993 | GCTGTCCTTTCTTCCAGACTACC |
PCR Primers
| Primer | Sequence |
| SU | CCACTCGTCTTCCGCATC |
| SD | TCAATGAAATTTGGCTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18077A-A6 | VGB6 | C57BL/6NTac | |
| 18077A-B11 | VGB6 | C57BL/6NTac | |
| 18077A-F10 | VGB6 | C57BL/6NTac | |
| 18077A-E5 | VGB6 | C57BL/6NTac | |
| 18077A-B8 | VGB6 | C57BL/6NTac | |
