| Gene | Chmp4c |
| Chromosome | 3 |
| Deletion Start | 10,390,043 |
| Deletion End | 10,367,035 |
| Deletion Size | 23,009 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,367,309 | 10,367,328 | GGACTGCTGACTTGCCTTTC |
| TUP | 10,367,329 | 10,367,353 | TAGGAACCGACCTGATCTCCAGGGC |
| TUR | 10,367,359 | 10,367,382 | TGCCTAGCTTAGCTCACTTCAATC |
| TDF | 10,389,439 | 10,389,462 | CCACTGGGTTCCCACATATCAATC |
| TDP | 10,389,467 | 10,389,496 | AGCAGATGTTCTCCTTGTTGTGTTTAGGGA |
| TDR | 10,389,501 | 10,389,523 | CAGAGCCAATGTTTCAGGAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TCTTTGAGCCACTTTCTCGG |
| SD | ACACAGCTGCCTCGTATTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18078B-C8 | VGB6 | C57BL/6NTac | |
| 18078B-E9 | VGB6 | C57BL/6NTac | |
| 18078B-H8 | VGB6 | C57BL/6NTac | |
