| Gene | Eif5a |
| Chromosome | 11 |
| Deletion Start | 69,919,312 |
| Deletion End | 69,917,433 |
| Deletion Size | 1,880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,918,604 | 69,918,622 | AAGGCGTGCTCCTCTTCTG |
| TUP | 69,918,575 | 69,918,599 | CCTTGCATACTGACTGCACAGTCCC |
| TUR | 69,918,485 | 69,918,505 | TTGGACAAACGTGGACAAGTG |
| TDF | 69,917,806 | 69,917,827 | TGGTAAAGGCTGTGGAGTGAAG |
| TDP | 69,917,775 | 69,917,800 | TGCTGTCACTGTAGTTGGTCTGTCCA |
| TDR | 69,917,708 | 69,917,728 | CCCATCCTGGATGCCAATCAG |
PCR Primers
| Primer | Sequence |
| SU | CATCTCTGGGATTTGGCATC |
| SD | AAGAGGCAGGGAGTTTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18082A-C1 | VGB6 | C57BL/6NTac | |
| 18082A-C5 | VGB6 | C57BL/6NTac | |
| 18082A-D1 | VGB6 | C57BL/6NTac | |
| 18082A-H5 | VGB6 | C57BL/6NTac | |
| 18082A-E8 | VGB6 | C57BL/6NTac | |
| 18082A-D5 | VGB6 | C57BL/6NTac | |
