18084 Myst3 Status: ES cell colonies screened / QC no positives
| Gene | Myst3 |
| Chromosome | 8 |
| Deletion Start | 22,862,796 |
| Deletion End | 22,862,205 |
| Deletion Size | 592 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,862,265 | 22,862,286 | AAGCAGAAACAGCGACCTTCAG |
| TUP | 22,862,287 | 22,862,312 | AGGAAAGGATCTGCAATGCCGTGTCC |
| TUR | 22,862,358 | 22,862,377 | GTCCCGTCTTTGACGCTCAG |
| TDF | 22,862,672 | 22,862,691 | TGGCCGACTCCTCAAAGATG |
| TDP | 22,862,696 | 22,862,720 | CCTCTACCGCCTCAACACTAAGGCA |
| TDR | 22,862,744 | 22,862,764 | AAACACGAGAGCGACTCACAG |
PCR Primers
| Primer | Sequence |
| SU | AGATGTGTGGAACCCAGGAG |
| SD | AGTAAGGTACTATAGAACCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
