| Gene | Polr3k |
| Chromosome | 2 |
| Deletion Start | 181,868,363 |
| Deletion End | 181,864,462 |
| Deletion Size | 3,902 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,864,481 | 181,864,498 | GCGGGAACGGACTCATTG |
| TUP | 181,864,510 | 181,864,532 | CAGCGTTGCCATCGCTTCGCCTG |
| TUR | 181,864,570 | 181,864,590 | GCACCCGTCTGTGGAACTTAC |
| TDF | 181,868,233 | 181,868,253 | GCCTACTTCATGCAGCTTCAG |
| TDP | 181,868,261 | 181,868,283 | CTGCAGACGAGCCAATGACCACC |
| TDR | 181,868,309 | 181,868,327 | TCCCTCCAGCGATGTCCAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCCTCATCACCCAGAACC |
| SD | TGACTATAAACCAAGTTCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18086A-F6 | VGB6 | C57BL/6NTac | |
| 18086A-H12 | VGB6 | C57BL/6NTac | |
| 18086A-C5 | VGB6 | C57BL/6NTac | |
| 18086A-E5 | VGB6 | C57BL/6NTac | |
| 18086A-F5 | VGB6 | C57BL/6NTac | |
