Gene | Raf1 |
Chromosome | 6 |
Deletion Start | 115,644,613 |
Deletion End | 115,619,233 |
Deletion Size | 25,381 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 115,644,508 | 115,644,530 | CCCTACCATTGTTCAGCAGTTTG |
TUP | 115,644,485 | 115,644,505 | TATCAGCGCCGGGCCTCAGAT |
TUR | 115,644,463 | 115,644,484 | AAGAATCCGTGAGCTTGCCATC |
TDF | 115,619,866 | 115,619,885 | ACCCGTTCAGCTTCCAGTCC |
TDP | 115,619,841 | 115,619,864 | ACGTGTACTCGTACGGCATCGTGC |
TDR | 115,619,794 | 115,619,813 | GTTGTTGATGTGGGCGTAGG |
PCR Primers
Primer | Sequence |
SU | CTTATACCTGAGGCGCGTTC |
SD | TAGTACCAAAGCAGGCTCCC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 68 bp | + | + | - |
TDF | TDR | 92 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
18087A-C4 | VGB6 | C57BL/6NTac | |
18087A-C6 | VGB6 | C57BL/6NTac | |
18087A-F11 | VGB6 | C57BL/6NTac | |
18087A-F2 | VGB6 | C57BL/6NTac | |
18087A-B12 | VGB6 | C57BL/6NTac | |