| Gene | Raf1 |
| Chromosome | 6 |
| Deletion Start | 115,644,613 |
| Deletion End | 115,619,233 |
| Deletion Size | 25,381 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,644,508 | 115,644,530 | CCCTACCATTGTTCAGCAGTTTG |
| TUP | 115,644,485 | 115,644,505 | TATCAGCGCCGGGCCTCAGAT |
| TUR | 115,644,463 | 115,644,484 | AAGAATCCGTGAGCTTGCCATC |
| TDF | 115,619,866 | 115,619,885 | ACCCGTTCAGCTTCCAGTCC |
| TDP | 115,619,841 | 115,619,864 | ACGTGTACTCGTACGGCATCGTGC |
| TDR | 115,619,794 | 115,619,813 | GTTGTTGATGTGGGCGTAGG |
PCR Primers
| Primer | Sequence |
| SU | CTTATACCTGAGGCGCGTTC |
| SD | TAGTACCAAAGCAGGCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18087A-C4 | VGB6 | C57BL/6NTac | |
| 18087A-C6 | VGB6 | C57BL/6NTac | |
| 18087A-F11 | VGB6 | C57BL/6NTac | |
| 18087A-F2 | VGB6 | C57BL/6NTac | |
| 18087A-B12 | VGB6 | C57BL/6NTac | |
