18089 2810408A11Rik Status: ES cell colonies screened / QC no positives
| Gene | 2810408A11Rik |
| Chromosome | 11 |
| Deletion Start | 69,900,748 |
| Deletion End | 69,897,410 |
| Deletion Size | 3,339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,899,843 | 69,899,861 | AGGAGAGGGTCGCCAGAAG |
| TUP | 69,899,817 | 69,899,842 | AGCTTCCTCCTTTGCTCCTTCCCTCA |
| TUR | 69,899,755 | 69,899,772 | TGTCAGCAGGGTGGTAGG |
| TDF | 69,898,169 | 69,898,188 | TGGGAGGACGGAACAAACTG |
| TDP | 69,898,140 | 69,898,167 | CCTCCTGTTACTTCCAGGTCCTTCAGGT |
| TDR | 69,898,104 | 69,898,122 | TGGAGTGCCTTTCGGATTC |
PCR Primers
| Primer | Sequence |
| SU | ACATCACAAGGCTCCTCACC |
| SD | TTGTGTTCTGAAGCAGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
