| Gene | Camta2 |
| Chromosome | 11 |
| Deletion Start | 70,686,374 |
| Deletion End | 70,670,150 |
| Deletion Size | 16,225 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,685,992 | 70,686,012 | GAGGAGGTGGAGTGCTATGTC |
| TUP | 70,685,969 | 70,685,991 | TGTGGACACTGGTTGCTGAGCAC |
| TUR | 70,685,913 | 70,685,937 | GACTCCCATAAGGTCCAAGACTTTG |
| TDF | 70,671,103 | 70,671,121 | GGCCTTGAGAGGCTCAGAC |
| TDP | 70,671,070 | 70,671,097 | CTCATTCTCTTCCTTTAGGGTCGGAGGC |
| TDR | 70,671,050 | 70,671,068 | TACCTCCTGCTGCTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTTCTTCTGAGCACCCTG |
| SD | AGGAGGAAACAGACGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18090A-H8 | VGB6 | C57BL/6NTac | |
