18091   Snrpa     Status: ES cell colonies screened / QC positives

GeneSnrpa
Chromosome7
Deletion Start27,195,531
Deletion End27,187,102
Deletion Size8,430
Genome Build38
MGI IDMGI:1855690
NCBI Gene ID53607
BAC ID224B17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF27,195,18227,195,202GGATCGTTCTTGCCATCAGTC
TUP27,195,15027,195,177TTCATGAGACTGTCTCTGTCCACTGACC
TUR27,195,10227,195,124CCTGTGTGTCATCTGCATTGTAG
TDF27,187,69727,187,715CAGGGCTTAGGCTGAGGAC
TDP27,187,67027,187,694AAGGTCCAGCCTGAGCTAGGGATCC
TDR27,187,62527,187,646TGTCACCAATCCCTACTCTGTC

PCR Primers

PrimerSequence
SUCTTAACTGGCTCCGACATCC
SDGCAACAGCTTTGTCCTCCTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR101 bp++-
TDFTDR91 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18091A-G9VGB6C57BL/6NTac
18091A-F11VGB6C57BL/6NTac
18091A-F2VGB6C57BL/6NTac
18091A-D11VGB6C57BL/6NTac
18091A-C10VGB6C57BL/6NTac
18091A-A3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image