| Gene | E430025E21Rik |
| Chromosome | 15 |
| Deletion Start | 59,370,062 |
| Deletion End | 59,332,236 |
| Deletion Size | 37,827 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,369,936 | 59,369,956 | CTGCTGTGTTCCTGCTGAAAG |
| TUP | 59,369,911 | 59,369,935 | ACCGGGCTGACCAGCAGAGATATGG |
| TUR | 59,369,865 | 59,369,889 | TCACAGGGAAAGCACCTTAAAGTAG |
| TDF | 59,332,496 | 59,332,516 | ACCAGCAATGAAAGGGTTGTC |
| TDP | 59,332,474 | 59,332,495 | TCGTTTCCCTATGCGCCTCAGC |
| TDR | 59,332,448 | 59,332,467 | TGGAGGAAGGTGAAGCACTG |
PCR Primers
| Primer | Sequence |
| SU | CCACAAGAGTACGGAAACTGG |
| SD | ACACGAGTTTACTGCTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18096A-A10 | VGB6 | C57BL/6NTac | |
| 18096B-D3 | VGB6 | C57BL/6NTac | |
| 18096B-E6 | VGB6 | C57BL/6NTac | |
| 18096B-F9 | VGB6 | C57BL/6NTac | |
