| Gene | Plekhg3 |
| Chromosome | 12 |
| Deletion Start | 76,578,489 |
| Deletion End | 76,560,171 |
| Deletion Size | 18,319 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,560,220 | 76,560,241 | GCCTAGTGTCCACCACTTCTTC |
| TUP | 76,560,243 | 76,560,265 | TCTGGCTCCTCCCGTGACAGTCA |
| TUR | 76,560,306 | 76,560,324 | GGGAGCCTGTCCCATTCTG |
| TDF | 76,578,334 | 76,578,355 | TCAGCAGACACAAACAAACAGG |
| TDP | 76,578,364 | 76,578,390 | AGAAACCTGAGGGAGAAGTTCCAGGCC |
| TDR | 76,578,396 | 76,578,417 | AGGAGTCAATCAACCCACAGAG |
PCR Primers
| Primer | Sequence |
| SU | CACTTATTTATCCCGGAGGTG |
| SD | TGACAAGGCAGTTAAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18097A-H11 | VGB6 | C57BL/6NTac | |
| 18097A-A12 | VGB6 | C57BL/6NTac | |
| 18097A-E4 | VGB6 | C57BL/6NTac | |
| 18097A-F2 | VGB6 | C57BL/6NTac | |
