| Gene | Psmg2 |
| Chromosome | 18 |
| Deletion Start | 67,654,022 |
| Deletion End | 67,641,682 |
| Deletion Size | 12,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,641,873 | 67,641,896 | GGCTGTCCGATTAGCATCTTTGTC |
| TUP | 67,641,897 | 67,641,922 | AGCCCTATCAAGGCCTGTCTGCTAGC |
| TUR | 67,641,924 | 67,641,943 | TCGCAGGCAACGAAAGTCTC |
| TDF | 67,653,902 | 67,653,921 | CATCTGCCCTGCCTTGGAAG |
| TDP | 67,653,926 | 67,653,946 | CCAGCTCTTGGCGGCTGCTCT |
| TDR | 67,653,987 | 67,654,008 | CAGCAGGATGAGGGAGAAACTG |
PCR Primers
| Primer | Sequence |
| SU | TAACTGTCTTCAAACGGTGCG |
| SD | TTAGGGTCTGTGGACTTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18104A-B1 | VGB6 | C57BL/6NTac | |
| 18104A-C2 | VGB6 | C57BL/6NTac | |
| 18104A-C8 | VGB6 | C57BL/6NTac | |
| 18104A-D9 | VGB6 | C57BL/6NTac | |
| 18104A-E2 | VGB6 | C57BL/6NTac | |
| 18104A-G3 | VGB6 | C57BL/6NTac | |
