| Gene | Syt3 |
| Chromosome | 7 |
| Deletion Start | 44,399,149 |
| Deletion End | 44,385,974 |
| Deletion Size | 13,176 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,386,600 | 44,386,619 | GTGTTGGTTGTGAGGCAGAG |
| TUP | 44,386,621 | 44,386,648 | CCAGAAAGCATGACTGTCCAGTGTTTCC |
| TUR | 44,386,652 | 44,386,672 | GGATGACCTGTGACCTGACTG |
| TDF | 44,398,713 | 44,398,732 | AAACTTCCAGTGAGCCACAC |
| TDP | 44,398,733 | 44,398,756 | TGCCACCACTAGGTGACACTGCAG |
| TDR | 44,398,783 | 44,398,801 | CAGCACCTTGGGCTGAAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGCAGTTTCCCACCCTAAG |
| SD | GAGGGAGCCTAACACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18106A-A10 | VGB6 | C57BL/6NTac | |
| 18106B-D1 | VGB6 | C57BL/6NTac | |
| 18106B-D3 | VGB6 | C57BL/6NTac | |
| 18106B-F11 | VGB6 | C57BL/6NTac | |
