| Gene | Txnl4b |
| Chromosome | 8 |
| Deletion Start | 109,572,944 |
| Deletion End | 109,568,969 |
| Deletion Size | 3,976 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,569,452 | 109,569,470 | GTGCGCTGCTCCTAGAATG |
| TUP | 109,569,471 | 109,569,497 | TGCCTTGTGGAGAAATCAGGTCTCACA |
| TUR | 109,569,515 | 109,569,538 | TCTCTTCACAGTTTCCCTCTTTAC |
| TDF | 109,572,579 | 109,572,599 | GTGGTCAGGCCATGTGTAGAG |
| TDP | 109,572,600 | 109,572,627 | AGACAAGAAGGGAGGAAGAGCCCACTAC |
| TDR | 109,572,649 | 109,572,667 | CAGAGCGTCCCAGTGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTCTTCCTACTCTGATGTGGC |
| SD | CACAAACATGGCAGAGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18107A-A7 | VGB6 | C57BL/6NTac | |
| 18107A-C4 | VGB6 | C57BL/6NTac | |
| 18107A-E5 | VGB6 | C57BL/6NTac | |
| 18107A-F5 | VGB6 | C57BL/6NTac | |
| 18107A-G10 | VGB6 | C57BL/6NTac | |
