| Gene | Esyt1 |
| Chromosome | 10 |
| Deletion Start | 128,525,820 |
| Deletion End | 128,510,564 |
| Deletion Size | 15,257 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,525,004 | 128,525,022 | TTCCGTTTCCTGCCTTGTC |
| TUP | 128,524,980 | 128,525,003 | TCTAGGTACCCGCTGAGACCCAGC |
| TUR | 128,524,924 | 128,524,944 | GCGACCGCGATAGGTCATTTC |
| TDF | 128,511,516 | 128,511,535 | GCGGAAGCTCGATGTCTCTG |
| TDP | 128,511,483 | 128,511,510 | TCAAACTCCTCCTTCATGTCACGAGAGC |
| TDR | 128,511,444 | 128,511,465 | GCTTTAGTCCTGGCCTTCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CGTGACCAGTCAAATACTGTC |
| SD | GAATGCCACTGCTAATGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18109A-B2 | VGB6 | C57BL/6NTac | |
| 18109A-B8 | VGB6 | C57BL/6NTac | |
| 18109A-B9 | VGB6 | C57BL/6NTac | |
| 18109A-D4 | VGB6 | C57BL/6NTac | |
| 18109A-F7 | VGB6 | C57BL/6NTac | |
| 18109A-H5 | VGB6 | C57BL/6NTac | |
