| Gene | Fbxo16 |
| Chromosome | 14 |
| Deletion Start | 65,299,413 |
| Deletion End | 65,270,740 |
| Deletion Size | 28,674 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,270,800 | 65,270,820 | TGGACACCTCTCAACCATCAG |
| TUP | 65,270,823 | 65,270,846 | TCTGAATGACCAGGTAGGTGGCCA |
| TUR | 65,270,859 | 65,270,878 | CCTCCTCACGGACCCAAATG |
| TDF | 65,298,791 | 65,298,812 | TCCGTCACAAAGCCCTAAACTC |
| TDP | 65,298,815 | 65,298,842 | TCCTCAGCACTAGATAAACCAGGCCAGA |
| TDR | 65,298,869 | 65,298,888 | CTTCCTGCCTCCATCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GAAACGCTTCTCTTTCTGGC |
| SD | GCACACACAGTAGCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18110A-B8 | VGB6 | C57BL/6NTac | |
| 18110A-G12 | VGB6 | C57BL/6NTac | |
| 18110A-H8 | VGB6 | C57BL/6NTac | |
| 18110A-C9 | VGB6 | C57BL/6NTac | |
| 18110A-D10 | VGB6 | C57BL/6NTac | |
