| Gene | Ppif |
| Chromosome | 14 |
| Deletion Start | 25,699,647 |
| Deletion End | 25,694,235 |
| Deletion Size | 5,413 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,694,629 | 25,694,648 | CGACGTTGGGCTTCCTAGAC |
| TUP | 25,694,649 | 25,694,670 | CCTCTGCGTCCGAGGTTGGACC |
| TUR | 25,694,702 | 25,694,721 | ACGTGAGCACCTGGCAACAC |
| TDF | 25,699,314 | 25,699,335 | ACAGAACTGGGAGCCTGGAATC |
| TDP | 25,699,339 | 25,699,363 | TGGAATGCAGCTGAAGAGCTCACAA |
| TDR | 25,699,376 | 25,699,400 | CAAAGGAACACCACTACCCTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGGTCACCTTGTGGGTGTC |
| SD | GGTGTCTGTGGCTTGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18113A-G1 | VGB6 | C57BL/6NTac | |
| 18113A-F9 | VGB6 | C57BL/6NTac | |
| 18113A-E8 | VGB6 | C57BL/6NTac | |
| 18113A-D2 | VGB6 | C57BL/6NTac | |
