| Gene | Tex22 |
| Chromosome | 12 |
| Deletion Start | 113,088,993 |
| Deletion End | 113,075,284 |
| Deletion Size | 13,710 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,076,129 | 113,076,149 | GCCAGGTCTGTCTCCTGTTTC |
| TUP | 113,076,150 | 113,076,173 | CTCCCACAGAGATCCTGGCCTAGC |
| TUR | 113,076,197 | 113,076,214 | AAGGCTCAGGCTGGGAAG |
| TDF | 113,088,869 | 113,088,889 | GACCTCAAGATGGCCTCACTC |
| TDP | 113,088,891 | 113,088,915 | TAAGAAGCAGCTCTGCTCAGCCCAG |
| TDR | 113,088,928 | 113,088,948 | CCACCACAAAGAAAGGCACTG |
PCR Primers
| Primer | Sequence |
| SU | GTGGGAAATGAGAGCTCAGG |
| SD | CAGGATCTCACCACCTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18114A-B11 | VGB6 | C57BL/6NTac | |
| 18114A-C2 | VGB6 | C57BL/6NTac | |
| 18114A-B8 | VGB6 | C57BL/6NTac | |
| 18114A-E1 | VGB6 | C57BL/6NTac | |
| 18114A-A12 | VGB6 | C57BL/6NTac | |
