| Gene | Xbp1 |
| Chromosome | 11 |
| Deletion Start | 5,524,955 |
| Deletion End | 5,520,998 |
| Deletion Size | 3,958 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,521,382 | 5,521,402 | CCGACACAGGCACAGAAGAAG |
| TUP | 5,521,406 | 5,521,427 | CCCGACGCCCAGTCCTCTGTAA |
| TUR | 5,521,435 | 5,521,457 | TCCTACCCTACGACCACAAAGAG |
| TDF | 5,524,309 | 5,524,327 | AGGTGCAGGCCCAGTTGTC |
| TDP | 5,524,333 | 5,524,361 | CCCAGAACATCTTCCCATGGACTCTGACA |
| TDR | 5,524,397 | 5,524,417 | CACACGCCACTTAGGCTACAG |
PCR Primers
| Primer | Sequence |
| SU | GGACTCCAGCAACCAGTG |
| SD | TAGGCAATGTGATGGTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18115A-H7 | VGB6 | C57BL/6NTac | |
| 18115A-C11 | VGB6 | C57BL/6NTac | |
| 18115A-A9 | VGB6 | C57BL/6NTac | |
| 18115A-F6 | VGB6 | C57BL/6NTac | |
