| Gene | Sec61a2 |
| Chromosome | 2 |
| Deletion Start | 5,895,260 |
| Deletion End | 5,871,779 |
| Deletion Size | 23,482 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,894,649 | 5,894,673 | CCCAGTCATTCTGTAGTGTTCATTG |
| TUP | 5,894,617 | 5,894,644 | CTGTAAGTTGGTGCGCAAGGTATGGACG |
| TUR | 5,894,550 | 5,894,574 | CTGTGTGAAAATACATGAGGGTGAG |
| TDF | 5,872,122 | 5,872,140 | TGAGGACTGCTCCGACAAG |
| TDP | 5,872,086 | 5,872,114 | TGAGGGAATCATTTCTTGCAAAACTGTGC |
| TDR | 5,872,042 | 5,872,061 | GTCAGGCACTGGGAAACAAG |
PCR Primers
| Primer | Sequence |
| SU | GTCCTGGAGTAGAGCAAC |
| SD | CAGGCTGAGTGGCAGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18118A-G9 | VGB6 | C57BL/6NTac | |
| 18118A-E3 | VGB6 | C57BL/6NTac | |
