| Gene | Tob1 |
| Chromosome | 11 |
| Deletion Start | 94,214,836 |
| Deletion End | 94,213,643 |
| Deletion Size | 1,194 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,213,875 | 94,213,895 | TTGACGATGTTCGTGGCAATC |
| TUP | 94,213,896 | 94,213,922 | TGCCACAGGATTTGAGTGTCTGGATCG |
| TUR | 94,213,934 | 94,213,953 | TCGCCGATCTGGTAGGAAAC |
| TDF | 94,214,246 | 94,214,264 | CGCACTTCTCCGATCAACC |
| TDP | 94,214,265 | 94,214,292 | TGGGCCTGACTGTAAATGTGAACGACCT |
| TDR | 94,214,302 | 94,214,323 | CACTGAGGAAGAGATGGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCGGTCATTTCCAGTCTTTC |
| SD | GAAATGCAATACCATGTCGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18119A-C5 | VGB6 | C57BL/6NTac | |
| 18119A-H3 | VGB6 | C57BL/6NTac | |
| 18119A-H10 | VGB6 | C57BL/6NTac | |
