| Gene | Afg3l1 |
| Chromosome | 8 |
| Deletion Start | 123,502,087 |
| Deletion End | 123,477,913 |
| Deletion Size | 24,175 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,478,161 | 123,478,178 | TTCTGCCTCCGACTCCAC |
| TUP | 123,478,180 | 123,478,206 | AGTGTCTACCTACGGCTGATCGGAACA |
| TUR | 123,478,207 | 123,478,224 | AGATCGCACCCGCTCAAG |
| TDF | 123,501,519 | 123,501,541 | TGCAGTTTGGGATGAGTGAGAAG |
| TDP | 123,501,543 | 123,501,566 | TGGGCCAGGTGTCCTTTGACTTCC |
| TDR | 123,501,609 | 123,501,630 | TCATCAATGAGCTGGGCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | CATTGATGTCGTTGACAATGC |
| SD | TCAGCCCTTGAGCAGGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18122A-E12 | VGB6 | C57BL/6NTac | |
| 18122A-F11 | VGB6 | C57BL/6NTac | |
