Gene | Afg3l1 |
Chromosome | 8 |
Deletion Start | 123,502,087 |
Deletion End | 123,477,913 |
Deletion Size | 24,175 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 123,478,161 | 123,478,178 | TTCTGCCTCCGACTCCAC |
TUP | 123,478,180 | 123,478,206 | AGTGTCTACCTACGGCTGATCGGAACA |
TUR | 123,478,207 | 123,478,224 | AGATCGCACCCGCTCAAG |
TDF | 123,501,519 | 123,501,541 | TGCAGTTTGGGATGAGTGAGAAG |
TDP | 123,501,543 | 123,501,566 | TGGGCCAGGTGTCCTTTGACTTCC |
TDR | 123,501,609 | 123,501,630 | TCATCAATGAGCTGGGCAGTAG |
PCR Primers
Primer | Sequence |
SU | CATTGATGTCGTTGACAATGC |
SD | TCAGCCCTTGAGCAGGTTAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 64 bp | + | + | - |
TDF | TDR | 112 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
18122A-E12 | VGB6 | C57BL/6NTac | |
18122A-F11 | VGB6 | C57BL/6NTac | |