| Gene | Ahsa1 |
| Chromosome | 12 |
| Deletion Start | 87,273,823 |
| Deletion End | 87,266,845 |
| Deletion Size | 6,979 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,267,092 | 87,267,112 | CGATGGGTTCGACATGGACTG |
| TUP | 87,267,120 | 87,267,147 | CCCAAGGAGGAATTTCAGAGCGAGAACA |
| TUR | 87,267,148 | 87,267,167 | ATGCAGCAACTAGGGCACTC |
| TDF | 87,273,418 | 87,273,440 | GCCTCAGCAAACTGAACTCAATG |
| TDP | 87,273,450 | 87,273,479 | CCCGAGAAACACATTGCTATGAAGTGGAGG |
| TDR | 87,273,503 | 87,273,521 | CAATGGCAGCAAGCGTTAC |
PCR Primers
| Primer | Sequence |
| SU | GCTCAGCAAGGTCGGTTTAG |
| SD | CAGACTGAATTACCTACACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18123A-B11 | VGB6 | C57BL/6NTac | |
| 18123A-G12 | VGB6 | C57BL/6NTac | |
| 18123A-F1 | VGB6 | C57BL/6NTac | |
| 18123A-C6 | VGB6 | C57BL/6NTac | |
| 18123A-G11 | VGB6 | C57BL/6NTac | |
