| Gene | Hsph1 |
| Chromosome | 5 |
| Deletion Start | 149,636,073 |
| Deletion End | 149,617,506 |
| Deletion Size | 18,568 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 149,635,667 | 149,635,687 | GAGCGAGCGGACTGTTGTTAG |
| TUP | 149,635,641 | 149,635,666 | CGACCACAGAAACACGCTATGGTGGA |
| TUR | 149,635,620 | 149,635,639 | GACGCAGAGACGGAGGATTC |
| TDF | 149,618,384 | 149,618,403 | TCAGAGCGAAGGTCAAGGTG |
| TDP | 149,618,361 | 149,618,383 | AGTGGTGCCAGCTACCGACTGCA |
| TDR | 149,618,341 | 149,618,358 | GGTGTGCCAAGCTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | GAATCTTTCTCGAAGGCTCGAG |
| SD | CCCTTGGCAAGAATAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18125A-E3 | VGB6 | C57BL/6NTac | |
| 18125A-H9 | VGB6 | C57BL/6NTac | |
