| Gene | Mafk |
| Chromosome | 5 |
| Deletion Start | 139,800,591 |
| Deletion End | 139,799,186 |
| Deletion Size | 1,406 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,799,523 | 139,799,544 | CCCTCCATCCCAGGTAAACATC |
| TUP | 139,799,546 | 139,799,570 | TCAGGTAAGCTGGTGTCCCTTCTGC |
| TUR | 139,799,580 | 139,799,601 | TGGTGATAGCGACATCGAGTAG |
| TDF | 139,800,030 | 139,800,049 | CCCGTCCCATGAACTCTGTG |
| TDP | 139,800,051 | 139,800,072 | CCCTGTTCTGACCGCTGCTCGT |
| TDR | 139,800,118 | 139,800,139 | ACACCAGCTCATCATCGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGCTCCAATGTGCTGAAG |
| SD | CTAAAGCAGGTTGTAGCGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18126A-A10 | VGB6 | C57BL/6NTac | |
| 18126A-D10 | VGB6 | C57BL/6NTac | |
| 18126A-H7 | VGB6 | C57BL/6NTac | |
| 18126A-E2 | VGB6 | C57BL/6NTac | |
