| Gene | Pdcd6 |
| Chromosome | 13 |
| Deletion Start | 74,317,204 |
| Deletion End | 74,303,915 |
| Deletion Size | 13,290 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,316,792 | 74,316,812 | TGGCTGCTATTGCATGTCTTC |
| TUP | 74,316,770 | 74,316,791 | TGTCCCTGGCCGTCCTTCTGAA |
| TUR | 74,316,739 | 74,316,758 | TGGCACGAGTCAGTGGAAAG |
| TDF | 74,304,653 | 74,304,675 | AACTCTATCGTGGAGGCAAGTAG |
| TDP | 74,304,619 | 74,304,643 | AATTCAAGGCCAGCCTGGTCTGGTC |
| TDR | 74,304,575 | 74,304,595 | GACAGGGTCCCACTGTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGGCGTGGTCTCTTCTGAG |
| SD | AAGCCTTCTGTGCAAGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18129A-G3 | VGB6 | C57BL/6NTac | |
| 18129A-C2 | VGB6 | C57BL/6NTac | |
