| Gene | Pi4kb |
| Chromosome | 3 |
| Deletion Start | 95,005,947 |
| Deletion End | 94,984,014 |
| Deletion Size | 21,934 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,984,745 | 94,984,763 | GAAGCCAGCTCACCGAAAG |
| TUP | 94,984,764 | 94,984,788 | AGGGAGCTGCCGACATTAAGCCCAG |
| TUR | 94,984,834 | 94,984,854 | TGGCCGTAGCATCTGACTTAG |
| TDF | 95,005,362 | 95,005,383 | CCAGGTCATCTGTGAGTGTCTG |
| TDP | 95,005,387 | 95,005,410 | TAGCATAGGCAGAGGCCTGCAACC |
| TDR | 95,005,427 | 95,005,446 | GGGAAGAGCCTCTGGGAATG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGTAAGACTTGAGTTGATCCC |
| SD | GTTCTCTGGAGGGCAGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18131A-H11 | VGB6 | C57BL/6NTac | |
| 18131A-B5 | VGB6 | C57BL/6NTac | |
| 18131A-D12 | VGB6 | C57BL/6NTac | |
| 18131A-A2 | VGB6 | C57BL/6NTac | |
