| Gene | Zfp346 |
| Chromosome | 13 |
| Deletion Start | 55,132,483 |
| Deletion End | 55,105,347 |
| Deletion Size | 27,137 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,105,919 | 55,105,937 | GCGACGCCTCTCACTGTTC |
| TUP | 55,105,938 | 55,105,960 | CAACTCGTGGTGGACCTCCTGCC |
| TUR | 55,105,976 | 55,105,996 | CTCACGTTGGTCCTCCCTCTC |
| TDF | 55,132,340 | 55,132,359 | GACGCCAGTGCAAACACAAC |
| TDP | 55,132,360 | 55,132,386 | CCACTCCAAAGGACTCAAGCACAGTGC |
| TDR | 55,132,391 | 55,132,410 | CCAGGCAGGGACAGCTTTAG |
PCR Primers
| Primer | Sequence |
| SU | CTAAGGCGCCAGAGGAACC |
| SD | CTGGGAGACTTCATGGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18132A-C2 | VGB6 | C57BL/6NTac | |
| 18132B-C10 | VGB6 | C57BL/6NTac | |
