| Gene | Ciao1 |
| Chromosome | 2 |
| Deletion Start | 127,247,617 |
| Deletion End | 127,242,731 |
| Deletion Size | 4,887 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,247,156 | 127,247,175 | CTGTTCTCTGCTTGCAGGTG |
| TUP | 127,247,125 | 127,247,154 | CAGCTGGATTTGCAAGTCTGTCCTTTCTGA |
| TUR | 127,247,088 | 127,247,108 | GGACCAGGCCACTTTCCTAAC |
| TDF | 127,242,979 | 127,243,000 | GATGATGCCATCCGAGTGTTTG |
| TDP | 127,242,956 | 127,242,978 | AGGAAGACCCAGGCTCAGACCCA |
| TDR | 127,242,928 | 127,242,947 | GGGCTGTCAGAGAGAAGGTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGGCTTCTTAGCTGCCC |
| SD | CTAGAATTGGGACAGTGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18133A-A5 | VGB6 | C57BL/6NTac | |
| 18133A-A12 | VGB6 | C57BL/6NTac | |
| 18133A-F12 | VGB6 | C57BL/6NTac | |
| 18133A-C1 | VGB6 | C57BL/6NTac | |
| 18133A-E7 | VGB6 | C57BL/6NTac | |
| 18133A-B5 | VGB6 | C57BL/6NTac | |
