| Gene | Fam108c |
| Chromosome | 7 |
| Deletion Start | 84,151,820 |
| Deletion End | 84,114,254 |
| Deletion Size | 37,567 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,151,232 | 84,151,252 | GCTTGTGCTCCCGGATTGTTG |
| TUP | 84,151,207 | 84,151,231 | CATTCGCTCGAGATTGCAACAGCCC |
| TUR | 84,151,167 | 84,151,188 | AGCAGGCAGGACCCTTTAAGTC |
| TDF | 84,115,029 | 84,115,049 | AGCTACGCCTGCTGCTTGTTC |
| TDP | 84,115,001 | 84,115,027 | CTAACATCTGCACTTGGCTGGAGCTGC |
| TDR | 84,114,981 | 84,114,998 | ACTGCACTCCCAGCCTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCGTTCCGATGGGTAGC |
| SD | CAGGACATGCTGCATGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18134A-C12 | VGB6 | C57BL/6NTac | |
| 18134A-G2 | VGB6 | C57BL/6NTac | |
| 18134A-G9 | VGB6 | C57BL/6NTac | |
