| Gene | Myoz1 |
| Chromosome | 14 |
| Deletion Start | 20,655,345 |
| Deletion End | 20,649,366 |
| Deletion Size | 5,980 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,655,055 | 20,655,075 | GGAAGAACTCAGCATCCACTC |
| TUP | 20,655,030 | 20,655,054 | TGCATCCTCCCATCCTTCCCATTGA |
| TUR | 20,655,003 | 20,655,024 | TCTTAGCAGGGTGTTGTCTCAG |
| TDF | 20,650,033 | 20,650,056 | GGTTGAGGAAGGTCTACAGAAGTG |
| TDP | 20,650,000 | 20,650,028 | TGCATTTGATGGTTAAGTCTTCCAGCCAC |
| TDR | 20,649,966 | 20,649,990 | ACAACTCCAACTCTAAGGTCATCTC |
PCR Primers
| Primer | Sequence |
| SU | GGGAAGCCTAAGGAGGAGAC |
| SD | TCAGCCTTTGTACCTCGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18136A-H3 | VGB6 | C57BL/6NTac | |
| 18136A-F8 | VGB6 | C57BL/6NTac | |
| 18136A-E2 | VGB6 | C57BL/6NTac | |
| 18136A-G1 | VGB6 | C57BL/6NTac | |
