| Gene | Rnf167 |
| Chromosome | 11 |
| Deletion Start | 70,651,278 |
| Deletion End | 70,647,872 |
| Deletion Size | 3,407 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,648,024 | 70,648,044 | TGCAGACTTCGGAGCACAATG |
| TUP | 70,648,046 | 70,648,070 | CAGCATGGACTTTGCAGACCTTCCA |
| TUR | 70,648,085 | 70,648,105 | AGTCCCTCGTCGCTCAGAGTG |
| TDF | 70,651,037 | 70,651,059 | AGAGCAAGAGGAAGGCGATGAAG |
| TDP | 70,651,063 | 70,651,085 | AGCCAAGGGACCAACCTGCTTCA |
| TDR | 70,651,117 | 70,651,136 | TCCAAAGGAGGTGGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTATTCTGTCCTATTTCTG |
| SD | ACCAGAGAATGGGTCCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18138A-F1 | VGB6 | C57BL/6NTac | |
| 18138A-C12 | VGB6 | C57BL/6NTac | |
| 18138A-G4 | VGB6 | C57BL/6NTac | |
| 18138A-C11 | VGB6 | C57BL/6NTac | |
| 18138A-F6 | VGB6 | C57BL/6NTac | |
