| Gene | Zbtb1 |
| Chromosome | 12 |
| Deletion Start | 76,387,411 |
| Deletion End | 76,385,245 |
| Deletion Size | 2,167 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,385,504 | 76,385,523 | TGACCGCTCCTTCCAGTTTC |
| TUP | 76,385,530 | 76,385,557 | TTCAAAGTGGCAATGAACTACCTCCAGC |
| TUR | 76,385,581 | 76,385,603 | CAGTCTGCGTCCTGTATGTCTTC |
| TDF | 76,386,614 | 76,386,633 | TCAAGGGCAGACAGCTTCAG |
| TDP | 76,386,637 | 76,386,660 | CATGCTCAGAGGTGCGGAGAACCC |
| TDR | 76,386,675 | 76,386,694 | CAGCGTTCCCTAGCCCATTC |
PCR Primers
| Primer | Sequence |
| SU | AATGTGTGTTTCTGGTGGTCC |
| SD | GGAGTGGTCAATAATCTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18140A-D9 | VGB6 | C57BL/6NTac | |
| 18140B-G5 | VGB6 | C57BL/6NTac | |
| 18140B-H1 | VGB6 | C57BL/6NTac | |
| 18140B-H2 | VGB6 | C57BL/6NTac | |
