| Gene | Def8 |
| Chromosome | 8 |
| Deletion Start | 123,461,624 |
| Deletion End | 123,447,801 |
| Deletion Size | 13,824 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,447,954 | 123,447,977 | CAACTTAGATCAACCCTGTCACTC |
| TUP | 123,447,979 | 123,448,002 | TCCTGCTTGTAGCCCTTCCAGTGG |
| TUR | 123,448,014 | 123,448,034 | CCTCAGAGCAGGTGGAATGTG |
| TDF | 123,461,508 | 123,461,529 | GGCTCACATTGAGGAAGCAGTC |
| TDP | 123,461,534 | 123,461,559 | TTCCAGGAACCTGGCCTAGACATGGA |
| TDR | 123,461,566 | 123,461,583 | TGGCGTTCTCCAGTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTCTTGAGGCCTAGCACAG |
| SD | GGAGCAAGGCCATCTCTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18141A-A4 | VGB6 | C57BL/6NTac | |
| 18141A-A12 | VGB6 | C57BL/6NTac | |
| 18141A-F1 | VGB6 | C57BL/6NTac | |
| 18141A-E5 | VGB6 | C57BL/6NTac | |
| 18141A-B1 | VGB6 | C57BL/6NTac | |
