| Gene | Ing5 |
| Chromosome | 1 |
| Deletion Start | 93,818,330 |
| Deletion End | 93,804,006 |
| Deletion Size | 14,325 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,804,720 | 93,804,738 | GTGGGACTCCAAGGTGAAC |
| TUP | 93,804,740 | 93,804,769 | ATCTTTATTCTGATGTCCATCCAGTGGGCA |
| TUR | 93,804,787 | 93,804,808 | TCAGCAGTGAAATGAACTCTCC |
| TDF | 93,817,492 | 93,817,513 | GTTTGTGGGATTTCCAGGTGAG |
| TDP | 93,817,516 | 93,817,539 | AGTCCAACCGCACTGGCAAAGCTG |
| TDR | 93,817,569 | 93,817,587 | ACATGAGGCGGCTGTGAGG |
PCR Primers
| Primer | Sequence |
| SU | CATGGAAGTTAACCGATGCC |
| SD | ACAGACAGTCCTGGAAACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18142B-C10 | VGB6 | C57BL/6NTac | |
| 18142B-B3 | VGB6 | C57BL/6NTac | |
| 18142B-D10 | VGB6 | C57BL/6NTac | |
