| Gene | Nphp1 |
| Chromosome | 2 |
| Deletion Start | 127,788,851 |
| Deletion End | 127,757,683 |
| Deletion Size | 31,169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,788,668 | 127,788,689 | AAAGCCTTGCGGGTCAACTAAG |
| TUP | 127,788,639 | 127,788,663 | CGGTCTAACGACTCACCAACCCTCA |
| TUR | 127,788,613 | 127,788,632 | ACAAGGGCCTGAGGGATGAG |
| TDF | 127,758,336 | 127,758,353 | CCCTGTAGGGCACAGTTC |
| TDP | 127,758,303 | 127,758,331 | TTAGGGACTTGAGACAATGGCAAGGGAAC |
| TDR | 127,758,253 | 127,758,273 | TGCACTCCAGTGGGTTATTTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTTCAGAAAGCTGTGTAG |
| SD | GAGCACAGTGAAGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18144A-G11 | VGB6 | C57BL/6NTac | |
