| Gene | Nr4a1 |
| Chromosome | 15 |
| Deletion Start | 101,274,287 |
| Deletion End | 101,270,088 |
| Deletion Size | 4,200 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,270,607 | 101,270,627 | TGGACAGAGCAGTTGCCTAAG |
| TUP | 101,270,630 | 101,270,651 | TTCTTCAGGGCCTCCGCCACCT |
| TUR | 101,270,657 | 101,270,678 | GGGAGGACTGAAGGAGAAGAAG |
| TDF | 101,274,057 | 101,274,077 | TCTGAAGGAGCACATGGCTAC |
| TDP | 101,274,080 | 101,274,099 | TGGCAGGAGACCCACAGCCG |
| TDR | 101,274,126 | 101,274,145 | TCCGAAGCTCAGGCAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GACAGCCTTTCCTAGCTCC |
| SD | ATGGAAGGAGAGCGGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18145A-A6 | VGB6 | C57BL/6NTac | |
| 18145A-A10 | VGB6 | C57BL/6NTac | |
| 18145A-C10 | VGB6 | C57BL/6NTac | |
| 18145A-E5 | VGB6 | C57BL/6NTac | |
