| Gene | Acot8 |
| Chromosome | 2 |
| Deletion Start | 164,804,814 |
| Deletion End | 164,792,840 |
| Deletion Size | 11,975 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,804,734 | 164,804,753 | ACCTCCGTAGTGTGCTGGTC |
| TUP | 164,804,711 | 164,804,733 | ACGAGCGTGCTCAACCTCGAGCC |
| TUR | 164,804,655 | 164,804,673 | GGGTGCGAGCCTAGTTCAG |
| TDF | 164,793,099 | 164,793,119 | GTTCCAGTGTGCGGTTGTATG |
| TDP | 164,793,072 | 164,793,095 | ACAGCTCTGCCCTGTGTCAGATCC |
| TDR | 164,793,012 | 164,793,035 | TGTAAGCAAGGAGAAGGTGAGATG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGAGTGGCTTACAGGGC |
| SD | CTCCTGTCTTAAGGCCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18147A-F5 | VGB6 | C57BL/6NTac | |
| 18147A-E6 | VGB6 | C57BL/6NTac | |
| 18147A-E12 | VGB6 | C57BL/6NTac | |
| 18147A-A9 | VGB6 | C57BL/6NTac | |
