| Gene | Bnip3l |
| Chromosome | 14 |
| Deletion Start | 67,008,781 |
| Deletion End | 66,987,650 |
| Deletion Size | 21,132 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,008,044 | 67,008,063 | TGGCCTCACTCGTTGTGTTG |
| TUP | 67,008,015 | 67,008,042 | TGAGTGACCTGATCCCTCCTACTTTCCA |
| TUR | 67,007,988 | 67,008,005 | CGCCTGTCACCCAAGAAC |
| TDF | 66,987,853 | 66,987,871 | AGGGCTGTCCCAGCTTGTC |
| TDP | 66,987,824 | 66,987,847 | AGATCTCCCTATGGGCCAGGCTGC |
| TDR | 66,987,774 | 66,987,797 | TGCTGTAAGGAAGCAGAGTGTATC |
PCR Primers
| Primer | Sequence |
| SU | TCCAATGCCAACTTCCTAGC |
| SD | TTGCAAACCTGACCTCATTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18148A-C7 | VGB6 | C57BL/6NTac | |
| 18148A-E10 | VGB6 | C57BL/6NTac | |
