| Gene | Cln8 |
| Chromosome | 8 |
| Deletion Start | 14,896,899 |
| Deletion End | 14,894,691 |
| Deletion Size | 2,209 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,895,170 | 14,895,191 | GACCACGTTGCTCCTAGAGATG |
| TUP | 14,895,194 | 14,895,218 | CACGCCCTTCACCTGCATTTCCTGG |
| TUR | 14,895,225 | 14,895,243 | GGACCGCACTCACCTTCAG |
| TDF | 14,896,153 | 14,896,171 | GCTCGTGGCATGGTGTTAC |
| TDP | 14,896,178 | 14,896,204 | TCCGATTTAACCTGCATCCAAGGCCCT |
| TDR | 14,896,214 | 14,896,235 | TGCGTGACCTGGTTCTTGTTAG |
PCR Primers
| Primer | Sequence |
| SU | AACTAGGCTGCTGCGAAGAC |
| SD | AAATAAGGCACACCCACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18149A-A4 | VGB6 | C57BL/6NTac | |
| 18149A-A8 | VGB6 | C57BL/6NTac | |
| 18149A-D3 | VGB6 | C57BL/6NTac | |
