| Gene | Lbp |
| Chromosome | 2 |
| Deletion Start | 158,331,959 |
| Deletion End | 158,306,673 |
| Deletion Size | 25,287 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 158,306,876 | 158,306,898 | GGAGTCTGCTATACGGGTATGTG |
| TUP | 158,306,903 | 158,306,926 | TAGCTGCCATCTGGCTGGTCACTC |
| TUR | 158,306,938 | 158,306,957 | ACAAGCTATGGGCTGAGGTC |
| TDF | 158,331,592 | 158,331,614 | TGCAGCTTGGGAATGACAGATAG |
| TDP | 158,331,617 | 158,331,643 | AGGGTGAGAAATGGACACTGACTCTGC |
| TDR | 158,331,662 | 158,331,685 | CAGAAGATACCAGCGGTGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAATGGGAGTGGCTTATCC |
| SD | CCTTAGAGACCACAGCTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18151A-B6 | VGB6 | C57BL/6NTac | |
| 18151A-D3 | VGB6 | C57BL/6NTac | |
