| Gene | Lhx1 |
| Chromosome | 11 |
| Deletion Start | 84,524,172 |
| Deletion End | 84,519,528 |
| Deletion Size | 4,645 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,523,534 | 84,523,553 | GTCCCTGCCTCTTCCTTCTC |
| TUP | 84,523,499 | 84,523,526 | TCTTGTCGGAACTACAGAGCCCTTGTGG |
| TUR | 84,523,473 | 84,523,496 | CGGACTCTTTATTGTGGCTTTCTG |
| TDF | 84,519,882 | 84,519,903 | CGGAGGCAACTACGACTTCTTC |
| TDP | 84,519,858 | 84,519,880 | CGCAAGGACCGCCATCCTCTCAG |
| TDR | 84,519,822 | 84,519,842 | TGATGGCACAAAGGGTAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TATTTATTTACGTCCCGCCG |
| SD | AGCGCCTCTAGGAAGGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18152A-A2 | VGB6 | C57BL/6NTac | |
| 18152A-G10 | VGB6 | C57BL/6NTac | |
