| Gene | Nfkbid |
| Chromosome | 7 |
| Deletion Start | 30,428,241 |
| Deletion End | 30,424,449 |
| Deletion Size | 3,793 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,424,500 | 30,424,519 | CAGTGGTGCTGAGCCTAGTG |
| TUP | 30,424,521 | 30,424,545 | CGCAGGCTTGTCATCCCAGCTACTC |
| TUR | 30,424,560 | 30,424,581 | CCCAGGTAGGTCTTGATCCTTC |
| TDF | 30,427,325 | 30,427,344 | GGTTGAAGCGGGTGTATGAG |
| TDP | 30,427,352 | 30,427,377 | TGAGGCACGATCTGGGTTCAAAGACC |
| TDR | 30,427,412 | 30,427,432 | CATGGGCCTGTAACCACAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCAGGACTTCAGATCCCAG |
| SD | CCGAAAGGTACTCAGATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18154A-B1 | VGB6 | C57BL/6NTac | |
| 18154A-G10 | VGB6 | C57BL/6NTac | |
