| Gene | Plekhm1 |
| Chromosome | 11 |
| Deletion Start | 103,403,866 |
| Deletion End | 103,366,844 |
| Deletion Size | 37,023 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,403,451 | 103,403,472 | GGGCCTCAAACTCACTCCATAG |
| TUP | 103,403,403 | 103,403,431 | TTTCCTGCTTTGACCTTCAGGGTTCTAGG |
| TUR | 103,403,382 | 103,403,402 | ACAGTGATTTGTGCCAGCAAC |
| TDF | 103,367,241 | 103,367,264 | TCAGTAGGTAAACCAGCCAGTATC |
| TDP | 103,367,216 | 103,367,240 | AGCTCTGGGCCTGGGCTCTTGTTTA |
| TDR | 103,367,183 | 103,367,207 | ACGTGACTATTCTAGCCTGTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGCTCTCCCTTCTCCTCG |
| SD | TCTTGGGTGACTTGGGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18155A-H9 | VGB6 | C57BL/6NTac | |
