| Gene | Zc3h12a |
| Chromosome | 4 |
| Deletion Start | 125,127,045 |
| Deletion End | 125,119,197 |
| Deletion Size | 7,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 125,126,332 | 125,126,351 | TGCCTGGAGTGTACCTTGTG |
| TUP | 125,126,304 | 125,126,330 | CATGGTCTATCCTTGCCTACGGCATGA |
| TUR | 125,126,251 | 125,126,269 | GCCCAACTGGCTAAGTGAC |
| TDF | 125,120,039 | 125,120,057 | GTGCCAACGCTCTCCTCTC |
| TDP | 125,120,013 | 125,120,038 | ACCTCCCAGGACTCCAGTCAAGGACA |
| TDR | 125,119,952 | 125,119,971 | GGTTCAGCCTCTAGGGACAC |
PCR Primers
| Primer | Sequence |
| SU | CTTGGGTCCCTTGGGTTG |
| SD | ACATGGTGCCTAACTAGCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18158A-A5 | VGB6 | C57BL/6NTac | |
| 18158A-C5 | VGB6 | C57BL/6NTac | |
| 18158A-F1 | VGB6 | C57BL/6NTac | |
| 18158A-D7 | VGB6 | C57BL/6NTac | |
| 18158A-B8 | VGB6 | C57BL/6NTac | |
